If someone has been planning recently to conduct a paternity test then this article will act as a quick guide. Paternity tests are something that used to establish whether the child is really the son or daughter of the man. Even to determine whether the man is the person who should be responsible for upbringing the child. It is not something new but as the technology has progressed so do the type of tests that helps to determine paternity in a fast and safe manner.
When it comes to paternity DNA testing, you will find that there are two large methods to perform the tests. What are those methods? –
- PCR or polymerase chain reaction
- RFLP or restriction fragment length polymorphism
Polymerase Chain Reaction
When it comes to real-time PCR, you will see that the testing usually involves the collection of a swab from the inner of the cheek of people for DNA samples. This test, however, does not provide the same level of information as an RFLP test can provide.
If you are conducting a PCR DNA test, you will find that it simply amplifies the DNA volume or simply take a small sample of DNA and then start copying it. This is useful if the researcher needs only a small sample and it is highly useful if someone is working with degraded DNA. With the use of real-time PCR in DNA testing, the laboratory needs to stay particular and careful to avoid contamination within the sample as the DNA amplification process can increase the chances of contamination.
The process of PCR DNA involves step-like heating of DNA, adding primers and then cooling it so that it can recombine. Then it allows the enzyme to read the DNA sequence in order to generate more than one copy of the original DNA.
Restriction Fragment Length Polymorphism
This test takes more time compared to PCR test. RFLP is a little old and traditional method, but the results it provides more reliable results as each locus provides more information regarding paternity and the result is more perfect as per the researchers. The test requires a larger sample of DNA and is more likely to use blood for DNA testing but one can also carry it out using a swab from the inner cheek if necessary.
Things to remember
- With the progress of technology, it has become difficult to fraud paternity testing, so you do not need to fear about accurate results.
- Paternity tests are performed to prove whether a man has a paternal obligation to the claimed son and daughter.
So after carrying out the ABO blood test, one must opt for the DNA testing which are quite specific steps to determine one’s paternity. And for DNA testing these two are the best methods discussed above.
Author Bio – Evans Walsh is a freelance content writer. He has written many good and informative articles on different categories such as technology, health, fashion, beauty, education, career, travel etc. He is very responsible towards her job. He loves to share his knowledge and experience with his friends and colleagues.